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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,182)
Mouse Secondary Antibodies (14,490)
Rabbit Secondary Antibodies (6,302)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,234)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (76)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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3,886 – 3,900 of 71,981 results
3,886

Goat anti-Rabbit IgM Heavy Chain Secondary Antibody, DyLight 405, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Goat
Conjugate DyLight 405
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline B Cell Development and Differentiation Markers, Immunology
Antigen IgM Heavy Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene ID (Entrez) 3507
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Rabbit IgM
Primary or Secondary Secondary
3,887

Donkey anti-Sheep IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Sheep
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Sheep IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human, mouse, and rabbit IgG.
3,888

Goat anti-Mouse IgG Fc Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Mouse
Host Species Goat
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Concentration 1.50mg/mL
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG, Fc fragment
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG
3,889

Chicken anti-Rabbit IgG (H+L) Secondary Antibody, TRITC (Pre-adsorbed), Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Rabbit
Host Species Chicken
Conjugate TRITC
Applications ELISA,Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20-1:2000, ELISA, Immunocytochemistry/Immunofluorescence, Immunomicroscopy 1:20-1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against human and mouse IgG.
3,890

Rabbit anti-Golden Syrian Hamster IgG F(ab')2 Secondary Antibody, FITC, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Golden Syrian Hamster
Host Species Rabbit
Conjugate Fluorescein
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Concentration LYOPH
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Hamster IgG F(ab')2 fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Hamster IgG F(c).
3,891

Rabbit anti-Equine IgG F(ab')2 Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Equine
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration 1 mg/mL
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2000, ELISA 1:2000 - 1:8000, Immunohistochemistry 1:200 - 1:1000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Horse IgG F(ab')2 fragment
Primary or Secondary Secondary
Test Specificity No reaction was observed against Horse IgG F(c).
3,892

Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody, DyLight 405, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Rabbit
Conjugate DyLight 405
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Isotype IgG2b
Concentration LYOPH
Antigen IgG2b Heavy Chain
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Mouse IgG2b chain
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity Minimal cross reactivity was noted against other Mouse immunoglobulin classes or subclasses.
3,893

Goat anti-Canine IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Canine
Host Species Goat
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Dog IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Dog IgG and Dog Serum.
3,894

Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 2 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:40000, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence, Fluorophore-linked immunosorbent assay
Classification Polyclonal
Immunogen Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with mouse IgG whole molecule in rabbit
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Human Serum Proteins.
3,895

Rabbit anti-Equine IgG Fc Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Equine
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration 2 mg/mL
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:2000000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Horse IgG F(c) fragment
Primary or Secondary Secondary
Test Specificity No reaction was observed against Horse IgG F(ab).
3,896

Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody, FITC, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Mouse
Host Species Rabbit
Conjugate Fluorescein
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG3
Concentration LYOPH
Antigen IgG3 Heavy Chain
Gene Symbols IGHG3
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Gene ID (Entrez) 3502
Classification Polyclonal
Immunogen Mouse IgG3 heavy chain
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity Specificity was confirmed by ELISA.
3,897

Rabbit anti-Bovine IgG Fc Secondary Antibody, Biotin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Bovine
Host Species Rabbit
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Rabbit anti-Bovine IgG Fc fragment Secondary Antibody was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Bovine IgG F(ab).
3,898

Rat anti-Rabbit IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™
SDP

Rat Polyclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Rabbit
Host Species Rat
Conjugate Fluorescein
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Human, Goat, and Mouse Serum Proteins. No reaction was observed against Goat, Human and Mouse Serum Proteins.
3,899

Rabbit anti-Chicken IgG Fc Secondary Antibody, Biotin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Chicken
Host Species Rabbit
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:20000, ELISA 1:250000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Chicken IgG F(c) fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Chicken IgG F(ab).
3,900

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 488
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins. This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.